Glossary entry (derived from question below)
French term or phrase:
HMW de grande taille
English translation:
high molecular weight species
Added to glossary by
Denise Pavao
Dec 28, 2017 17:29
6 yrs ago
3 viewers *
French term
United States
HMW de grande taille
French to English
Medical
Medical (general)
clinical trial
Hello out there!
This is part of a request for additional information regarding a clinical trial.
Concernant la substance active:
L'origine des HMW de grande taille pour le lot de substance active du Procédé X présente une polydispersité plus importante que le lot du Procédé Y
I was the thinking "high molecular weight" but then there should be something that that is modifying. Am I on the wrong track?
I would appreciate your thoughts!
Thank you.
This is part of a request for additional information regarding a clinical trial.
Concernant la substance active:
L'origine des HMW de grande taille pour le lot de substance active du Procédé X présente une polydispersité plus importante que le lot du Procédé Y
I was the thinking "high molecular weight" but then there should be something that that is modifying. Am I on the wrong track?
I would appreciate your thoughts!
Thank you.
Proposed translations
(English)
| 4 +1 | high molecular weight species |
Sue Davis
|
| 4 | high molecular weigh |
liz askew
|
| 4 | high-molecular-weight (HMW) proteins |
Drmanu49
|
Proposed translations
+1
1 hr
Selected
high molecular weight species
https://www.ncbi.nlm.nih.gov/pubmed/20039266
Disaggregation of high-molecular weight species during downstream processing to recover functional monomer.
Xu X1, Didio DM, Leister KJ, Ghose S.
Author information
Abstract
The use of chaotropic agents to recover functional monomeric material was investigated for the downstream purification of an Fc-fusion protein containing high levels of high-molecular weight (HMW) species. In batch studies, chaotropic agents irreversibly disaggregated a majority of the aggregated protein. An integrated processing mode, termed as on-column disaggregation, was developed in which the protein was captured on Protein A chromatography and then a chaotropic agent was used to simultaneously elute the bound protein and disaggregate the HMW species. On-column disaggregation process resulted in protein recoveries of >95% and aggregation reduction of approximately 50%. Analytical results are presented showing that the recovered monomeric material was comparable to the reference protein in biochemical, biophysical, and pharmacokinetic properties. The kinetic and molecular mechanisms governing protein aggregation and disaggregation will also be elucidated. For the Fc-fusion protein studied here, incorporation of the disaggregation strategy in both batch and on-column modes led to an increase of >10% in overall downstream yield.
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Note added at 1 hr (2017-12-28 19:17:57 GMT)
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http://pubs.acs.org/doi/abs/10.1021/bi00011a021?journalCode=...
Self-Assembly of DNA Oligomers into High Molecular Weight Species
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Note added at 1 hr (2017-12-28 19:19:25 GMT)
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species is a useful general term when the molecules in question and their nature are not specified
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Note added at 1 hr (2017-12-28 19:22:31 GMT)
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So my first ref. refers to proteins, my second REF. to DNA
Disaggregation of high-molecular weight species during downstream processing to recover functional monomer.
Xu X1, Didio DM, Leister KJ, Ghose S.
Author information
Abstract
The use of chaotropic agents to recover functional monomeric material was investigated for the downstream purification of an Fc-fusion protein containing high levels of high-molecular weight (HMW) species. In batch studies, chaotropic agents irreversibly disaggregated a majority of the aggregated protein. An integrated processing mode, termed as on-column disaggregation, was developed in which the protein was captured on Protein A chromatography and then a chaotropic agent was used to simultaneously elute the bound protein and disaggregate the HMW species. On-column disaggregation process resulted in protein recoveries of >95% and aggregation reduction of approximately 50%. Analytical results are presented showing that the recovered monomeric material was comparable to the reference protein in biochemical, biophysical, and pharmacokinetic properties. The kinetic and molecular mechanisms governing protein aggregation and disaggregation will also be elucidated. For the Fc-fusion protein studied here, incorporation of the disaggregation strategy in both batch and on-column modes led to an increase of >10% in overall downstream yield.
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Note added at 1 hr (2017-12-28 19:17:57 GMT)
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http://pubs.acs.org/doi/abs/10.1021/bi00011a021?journalCode=...
Self-Assembly of DNA Oligomers into High Molecular Weight Species
--------------------------------------------------
Note added at 1 hr (2017-12-28 19:19:25 GMT)
--------------------------------------------------
species is a useful general term when the molecules in question and their nature are not specified
--------------------------------------------------
Note added at 1 hr (2017-12-28 19:22:31 GMT)
--------------------------------------------------
So my first ref. refers to proteins, my second REF. to DNA
Note from asker:
| Thank you Sue! |
Peer comment(s):
| agree |
Helen Genevier
: HMW species seems a great solution to me, as there are so many possibilities besides protein and DNA. I'd precede it by "large" in case the author is referring to a subset of HMW species./You too, Sue :-)
1 day 15 hrs
|
|
Thanks Helen, and Happy New Year!
|
4 KudoZ points awarded for this answer.
Comment: "Thanks again, Sue, and Happy New Year!"
10 mins
high molecular weigh
https://hal.archives-ouvertes.fr/hal-00884773/document
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by G Branlard - 1985 - Cited by 33 - Related articles
1 Jan 1985 - L'observation des sous-unités gluténines de haut poids moléculaire a été faite par électrophorèse en milieu SDS sur 195 blés tendres, pour la plupart d'origine française, et 38 blés durs. On observe 18 ... Observation of high molecular weight (HMW) glutenin subunits has been carried out by SDS PAGE for.
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Note added at 10 mins (2017-12-28 17:40:25 GMT)
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sorry
weight
Translate this page
by G Branlard - 1985 - Cited by 33 - Related articles
1 Jan 1985 - L'observation des sous-unités gluténines de haut poids moléculaire a été faite par électrophorèse en milieu SDS sur 195 blés tendres, pour la plupart d'origine française, et 38 blés durs. On observe 18 ... Observation of high molecular weight (HMW) glutenin subunits has been carried out by SDS PAGE for.
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Note added at 10 mins (2017-12-28 17:40:25 GMT)
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sorry
weight
Note from asker:
| Thanks Liz, but am I not missing a noun? HMW molecule? HMW component. That's what has me a little perplexed. |
17 mins
high-molecular-weight (HMW) proteins
High-molecular-weight surface-exposed proteins of Haemophilus ...
https://www.ncbi.nlm.nih.gov/pubmed/8106776 - Traduire cette page
de GJ Noel - 1994 - Cité 30 fois - Autres articles
Because of similarities between filamentous hemagglutinin (FHA) adhesin of Bordetella pertussis and high-molecular-weight (HMW) proteins commonly expressed by NT H. influenzae, the role that HMW proteins play in determining NT H. influenzae-macrophage interactions was assessed. In tests with genetically ...
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Note added at 19 minutes (2017-12-28 17:48:52 GMT)
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or large HMW DNA molecules
HMW DNA extraction for long-reads on @nanopore or ... - Enseqlopedia
enseqlopedia.com/.../hmw-dna-extraction-long-reads-nanopore-10...
Traduire cette page
29 mars 2017 - The Quick Protocol, sounds faster than the now obsolete, Loman Protocol (sounds like a Joy Division album) is capable of generating incredibly large HMW DNA molecules with the record standing somewhere north of 900kb. It uses a modified Sambrook phenol-chloroform extraction/purification (read Cell: ...
https://www.ncbi.nlm.nih.gov/pubmed/8106776 - Traduire cette page
de GJ Noel - 1994 - Cité 30 fois - Autres articles
Because of similarities between filamentous hemagglutinin (FHA) adhesin of Bordetella pertussis and high-molecular-weight (HMW) proteins commonly expressed by NT H. influenzae, the role that HMW proteins play in determining NT H. influenzae-macrophage interactions was assessed. In tests with genetically ...
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Note added at 19 minutes (2017-12-28 17:48:52 GMT)
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or large HMW DNA molecules
HMW DNA extraction for long-reads on @nanopore or ... - Enseqlopedia
enseqlopedia.com/.../hmw-dna-extraction-long-reads-nanopore-10...
Traduire cette page
29 mars 2017 - The Quick Protocol, sounds faster than the now obsolete, Loman Protocol (sounds like a Joy Division album) is capable of generating incredibly large HMW DNA molecules with the record standing somewhere north of 900kb. It uses a modified Sambrook phenol-chloroform extraction/purification (read Cell: ...
Note from asker:
| Thank you Dr Manu! |
Discussion
22 show a significant loss of monomers while denatured oligomers and
HMWS become predominant" https://hal-ens.archives-ouvertes.fr/hal-01510479/document.)
https://en.wikipedia.org/wiki/Dispersity
The dispersity index, or formerly polydispersity index (PDI), or heterogeneity index, or simply dispersity (Đ), is a measure of the distribution of molecular mass in a given polymer sample. Đ (PDI) of a polymer is calculated: , where is the weight average molecular weight and is the number average molecular weight.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3598102/
by JK Leypoldt - 2013 - Cited by 8 - Related articles
Early clinical studies by Mistry (4,5) demonstrated that a high-molecular-weight glucose polymer osmotic agent, similar to icodextrin, was superior to other glucose polymer osmotic agents of lower molecular weight and higher polydispersity. Improved osmotic agents for long-dwell PD solutions have previously been ..